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The functionality described in this
topic is only available when you mark Show
Advanced Options.
Add Content
Select Probes from SNP
Once you have chosen Select Probes from SNP from the CGH probegroup wizard: Add Content step, you will be directed to the following Define Targets step. Complete the fields and selections on this screen to define the desired genes or genomic regions that you want covered in the design. When you submit the probe selection job, SureDesign searches the probes in the selected SNP probe database to locate the ones that cover your specified chromosomes of interest.
Follow the steps below to define your targets.
In the SNP Probes drop-down list, select the probegroup that you want SureDesign to use to as the probe source of SNP probes for the design. These are Agilent-designed probes that are evenly spaced across the genome. The options are:
· Genomic_SNP_100k - contains 100,000 SNP probes; available for array formats 2 x 400 K and 1 x 1 M
· Genomic_SNP_60k - contains 60,000 SNP probes; available for array formats 1 x 1 M, 2 x 400 and 4 x 180 K
· Genomic_SNP_30k - contains 30,000 SNP probes; available for array formats 8 x 60 K and 4 x 180 K
· Genomic_SNP_15k - contains 15,000 SNP probes; available for array format 8 x 60 K
Select check boxes to indicate chromosomes you want included in your SNP probes. Click Select All to mark the check boxes for all chromosomes, or Deselect All to clear all check boxes.
To submit the probegroup for probe selection:
Click Begin
Probe Selection.
A message box opens indicating the e-mail address where Agilent will
contact you when the probe selection job is complete. If desired,
you can enter additional e-mail addresses into the provided field.
Click OK in the notification message to submit the probegroup to SureDesign. Your submission is placed in the SureDesign job queue to await probe selection.
The wizard takes you to the Finalize step. Click Close to close the wizard.