The functionality described in this topic is only available when you mark Show Advanced Options.

HaloPlex advanced wizard:

Add/Review Content

Select Existing Probes

Select Probe Source

Select probes by Amplicon ID

This screen of the advanced HaloPlex wizard is for selecting specific probes from an existing design or probegroup by entering the Amplicon IDs of the desired probes. SureDesign will then search the probes in the existing design/probegroup to locate the ones that match the specified Amplicon IDs, and add those probes to the new design.

Screen 1 - Targets

In the Targets text area, enter the Amplicon IDs of the probes that you want to add to your design using either of the following approaches:

·        Type or paste the Amplicon IDs directly into the text area. List one Amplicon ID per line.

·        Click Upload to browse to a text file (*.txt) that lists the Amplicon IDs (one Amplicon ID per line).

The Amplicon IDs for an existing design are listed in the design's Amplicons.bed file. See Download design files for information on how to download this file for a particular design.

Click Next to continue to Screen 2.

Screen 2 - Target Summary

This screen provides a target summary that indicates the number of Amplicon IDs that you entered on the previous screen. Review the summary before proceeding. When you are finished, click Pick Probes. The program will search the selected probe source for probes with the Amplicon IDs that you entered.

Target Summary

To submit the design for probe picking:

1. Click Pick Probes.
   
   A message box opens indicating the e-mail address where Agilent will contact you when the probe selection job is complete. If desired, you can enter additional e-mail addresses into the provided field.

2. Click OK in the notification message to submit the design to SureDesign.
   
   Your submission is placed in the SureDesign job queue to await probe selection.
   
   The wizard takes you to the Add/Review Content screen. The new probegroup appears in the Probegroup Summary table. The # Targets column of the table lists Processing until the probe selection job is complete. Click the refresh icon to see updates to this column.
   
   You receive an e-mail from Agilent SureDesign notifying you when your job is complete. In order to finalize the design, you must wait until your probe selection job is complete.