The functionality described in this topic is only available when you mark Show Advanced Options.

SureSelect RNA advanced wizard:

Add/Review Content

Select Existing Probes

Select probes by Probe ID

Once you have completed the Select Probe Source step, you will be directed to the Select probes by probe ID screen. Complete the fields and selections in this step to designate the specific probe IDs you wish to use.

Screen 1 - Probe IDs

Enter the probe IDs in the text box provided. You can type or paste the probe IDs directly into the text area, or click Upload to browse to a text file (*.txt) that lists the probe IDs (one per line). Click the Example hyperlink to open a new window displaying an example list of Probe IDs. The Probe IDs for an existing design are listed in the design's probes.txt file. See Download design files for information on how to download this file for a particular design.

Click Next to continue to Screen 2.

Screen 2 - Target Summary

This screen provides a chance for editing the default probegroup name and selecting a boosting option.

Boosting

SureDesign can boost the number of copies for any GC-rich probes in the new probegroup. Boosting these probes can potentially improve their ability to capture target genomic fragments.

In the Boosting drop-down list, select one of the options described below, or leave the default option in place. The default option is the one Agilent recommends, and it is determined by the hybridization type selected in the Define Design step.

·        Optimized Performance XT – This option is the default when the hybridization type is set to Overnight or Not Specified. This boosting algorithm is specifically optimized for the hybridization conditions in the SureSelect XT library preparation protocol to deliver optimal sequencing uniformity and read depth across the full range of GC content percentages.

·        Optimized Performance XT HS2 - This option is the default when the hybridization type is set to 90 minutes or Not specified. This boosting algorithm is specifically optimized for the hybridization conditions in the SureSelect XT HS2 library preparation protocol to deliver optimal sequencing uniformity and read depth across the full range of GC content percentages.

·        No Boosting - SureDesign does not boost GC-rich probes when this option is selected.

Target Summary

This section lists the number of target identifiers entered on the previous screen.

To submit the design for probe selection:

1. Click Begin Probe Selection.
   
   A message box opens indicating the e-mail address where Agilent will contact you when the probe selection job is complete. If desired, you can enter additional e-mail addresses into the provided field.

2. Click OK in the notification message to submit the design to SureDesign.
   
   Your submission is placed in the SureDesign job queue to await probe selection.
   
   The wizard takes you to the Add/Review Content screen. The new probegroup appears in the Probegroup Summary table. The # Targets column of the table lists Processing until the probe selection job is complete. Click the refresh icon to see updates to this column.
   
   You receive an e-mail from Agilent SureDesign notifying you when your job is complete. In order to finalize the design, you must wait until your probe selection job is complete.

Click Close Design Wizard to return to the SureDesign Home page. You will see your Design listed in the Designs: In Progress table.