Download Bait Tiling results

 

Bait tiling creates baits that evenly cover selected genomic intervals or transcript sequences. Before you can download Bait Tiling results, you must first set up a Bait Tiling job and submit it to Agilent for processing. See Bait Tiling (SureSelect Target Enrichment) and Bait Tiling (SureSelect RNA Enrichment). When your job is ready, it appears in the Search Results pane on the Bait Tiling page with a status of Completed. See View Bait Tiling results.

  1. Click the Workspace tab. For the SureSelect Target Enrichment application type, you can also enter a collaboration.

  2. Set the application type to SureSelect Target Enrichment or SureSelect RNA Enrichment.

  3. Click Baits > Bait Tiling.

    The Bait Tiling page appears. A search results pane appears at the bottom of the page that lists your current Bait Tiling jobs.

  4. In the Actions column of the Search Results pane, next to the desired job, click Download.

    A File Download dialog box appears.

  1. Click Save.

    A Save As dialog box appears.

  2. Select a location for the downloaded file, then click Save.

    eArray downloads the Bait Tiling results in zip format to the selected location.

    The downloaded zip archive contains up to six separate files that correspond to the types of available results. See the table below for details. All files are tab-delimited text files that you can view with a word processing or spreadsheet program.

File

Result

Description

BaitTiling_sum or RNAEnrichment_sum

 

Design Summary

List of the parameters that were used in the Bait Tiling process. Also contains information of an overall nature about the set of baits produced by the process, including statistics on the length and number of baits. For the SureSelect Target Enrichment application type, this file also shows the number of bases of the targeted genome that are covered by the baits that are produced by the Bait Tiling process.

BaitTiling_tdt or RNAEnrichment_tdt

 

Design Details

List of the baits generated by the bait design process, with specific information about each bait, including its length, sequence, location, and the target from which it was derived.

  • Note: Regions of baits that are designed to repeat-masked regions of the genome appear in lower case characters.

BaitTiling_fate or RNAEnrichment_fate

 

Target Fate

 

List of the target intervals that were used in the Bait Tiling process, with information on which ones produced baits, and which did not. For the SureSelect Target Enrichment application type, this file also includes additional statistics for each interval, including the size of the interval, the total number of target bases that are covered by the baits that were designed to the interval, and the number of baits that were designed to the interval but rejected.

eArray validates each interval before it uses it in the bait tiling process. In the Status column, valid intervals have a status of Pass. If an interval is not valid, the reason appears in Status.

BaitTiling_bed or RNA Enrichment_bed

BED File

BED format track file that contains the genomic coordinates of each bait. You can load this file into a compatible genome browser to see bait locations graphically. You may need to change the extension of the file to .bed before it will load properly.

BaitTiling_Covered
RegionsDetails

Covered Regions File

(SureSelect Target Enrichment application type) List of the subintervals within each input genomic target interval to which the Bait Tiling process designed baits. Statistics for each subinterval appear. These statistics include the size of each subinterval and the number of baits that were designed to it, and the number of base pairs in each subinterval that are covered by at least one bait.

Bait Tiling_
UncoveredRegions
Details

Uncovered Regions File

(SureSelect Target Enrichment application type) List of the regions within each genomic target interval that were excluded from the Bait Tiling process. Information also appears about the size of each uncovered region and the reason that it was excluded.

See also

Learn about SureSelect RNA Enrichment libraries

Create baits by Bait Tiling (SureSelect Target Enrichment)

Create baits by Bait Tiling (SureSelect RNA Enrichment)