View Bait Tiling results

 

You can view a list of your current Bait Tiling jobs, and check the status of each. If the status of the job is Completed, you can view its detailed results.

  1. Click the Workspace tab. For the SureSelect Target Enrichment application type, you can also enter a collaboration.

  2. Set the application type to SureSelect Target Enrichment or SureSelect RNA Enrichment.

  3. Click Baits > Bait Tiling.

    The Bait Tiling page appears. A search results pane appears at the bottom of the page that lists your current Bait Tiling jobs.

  1. Find the desired job in the Search Results pane at the bottom of the page. Its status appears in the Status column.

    The list of jobs does not automatically update once it appears. Click Refresh to update the list to its most current form.

Status designations

Status

Description

In Queue

The Bait Tiling job has been submitted to the system, but no action has been taken on it yet.

Designing

The Bait Tiling process has begun.

Completed

The Bait Tiling job is finished, and the results are available for your use.

ERROR

The job was started, but there are problems. You must resubmit the job. eArray sends you an e-mail that tells you about the error. This e-mail can contain additional information about the error.

  1. If the status of a job is Completed, you can view its detailed results. In the actions column of the list of jobs, next to the desired completed job, click View Design.

    The job's Tiling Design Details page opens in a new window.

  1. To view any of the available types of results, click the desired tab at the top of the page. These results can be available:

Result

Description

Design Summary

List of the parameters that were used in the Bait Tiling process. This tab also contains information of an overall nature about the set of baits produced by the process, including statistics on the length and number of baits. For the SureSelect Target Enrichment application type, this tab also shows the number of bases of the targeted genome that are covered by the baits that were produced by the Bait Tiling process.

Design Details

List of the first 100 baits generated by the Bait Tiling process, with specific information about each bait, including its Bait ID, sequence, and length. Additional annotation can also appear. To view the complete set of baits, download the Bait Tiling results, then use a word processing or spreadsheet program to view the BaitTiling_tdt file.

In the Sequence column, regions of baits that have been designed to repeat-masked regions of the genome appear in lower case characters.

Target Fate

 

List of the target intervals that were submitted for Bait Tiling, with information on which ones produced baits, and which did not. For the SureSelect Target Enrichment application type, this file also includes additional statistics for each interval, including the size of the interval and the total number of target bases that are covered by the baits that were designed to the interval.

eArray validates each interval before it uses it in the bait tiling process. In the Status column, valid intervals have a status of Pass. If an interval is not valid, the reason appears in Status.

BED File

Preview of the first 100 data lines of the BED format track file produced by the Bait Tiling process. You can download this file and view it in a genome browser that accepts BED format files.

Covered Regions File

(SureSelect Target Enrichment application type) List of the subintervals within each input genomic target interval to which the Bait Tiling process designed baits. Statistics for each subinterval appear. These statistics include the size of each subinterval and the number of baits that were designed to it, and the number of base pairs in each subinterval that are covered by at least one bait.

Up to 100 subregions appear in this tab. To view the complete list, download the bait tiling results, then use a word processing or spreadsheet program to view the Bait Tiling_CoveredRegionsDetails file.

Uncovered Regions File

(SureSelect Target Enrichment application type) List of the regions within the genomic target intervals that were excluded from the Bait Tiling process.

Up to 100 uncovered regions appear in this tab, with information about the size of each region and the reason that it was excluded. To view the complete list of uncovered intervals, download the bait tiling results, then use a word processing or spreadsheet program to view the Bait Tiling_UncoveredRegionsDetails file.

  1. When you are finished viewing the results of the Bait Tiling job, click Close.

    eArray closes the window, and returns you to the Bait Tiling page

    You can take other actions on the jobs in the Search Results pane. Depending on the status of the job, you can download the results, create a bait group from the results, and delete the job.

See also

Create baits by Bait Tiling (SureSelect Target Enrichment)

Create baits by Bait Tiling (SureSelect RNA Enrichment)

Learn about SureSelect enrichment libraries