View bait details and statistics

After you search for baits, the search results let you view information about the returned baits:

This topic describes how to view baits based on bait searches. In addition, you can view baits when you view bait groups.

View bait sequences and statistics

You can view the sequence and ID information of selected baits, as well as results of statistical calculations based on their base composition.

Search for the desired baits.

A Search Results pane lists the baits that match your search criteria.
In the Search Results pane, select the bait(s) of interest. Use the following as a guide:
- To select an individual bait, mark the check box next to its Bait ID.
- To select all of the baits on the current page, mark the check box in the heading row of the Search Results table.
- To go to another page of results, click one of the Pages links. eArray remembers the baits that you select as you go from page to page.
- To select all of the baits on every page of the search results, mark Select Entire Result.

Click Show Statistics.

A list of the selected baits appears. ID and sequence information appears for each bait, along with results of statistical calculations based on the bait's sequence. The table below describes these statistics. The statistics that are available vary by application type.

Statistic	Description	Example
Length	The number of nucleotides in the bait	A particular bait contains 120 nucleotides. Length = 120.
G%	Percentage of bases in the bait sequence that are G bases	Same bait as above: 22 Gs; %G = 18.33%
C%	Percentage of bases in the bait sequence that are C bases	Same bait as above: 32 Cs; %C = 26.67%
A%	Percentage of bases in the bait sequence that are A bases	Same bait as above: 26 As; %A = 21.67%
T%	Percentage of bases in the bait sequence that are T bases	Same bait as above: 40 Ts; %T = 33.33%
GC%	Percentage of bases in the bait sequence that are, collectively, G and C bases	Same bait as above: 22 Gs and 32 Cs. %GC = 45%
PolyX	The longest homeomeric run (run of one type of base) in the bait sequence, represented as the number of bases in the run.	ATTAGTTTATG has a PolyX of 3 because it contains a run of three Ts. Same bait as above: contains a run of 6 Ts and a run of 3 Gs; therefore, the PolyX = 6.
FivePrimeAs	The length of any poly A sequence on the 5' end of the bait	AAAAAATTGCATTA FivePrimeAs = 6
BC_Score	Base Composition Score: A numeric value that defines the quality of the bait, based upon its base composition and distribution	BC_1 is the best, and BC_Poor is the worst.
MAX_SUBSEQ_TM	The highest calculated Tm of a moving window of 20 bp regions within the bait
DELTAG	The calculated ΔG of self-folding. This indicates the likelihood that the bait will fold in on itself, which can affect both the bait manufacturing process as well as the pull-down of the desired genomic fragments.

To download the IDs, sequences, and statistics that appear in the window, follow these steps:

1. Click Download.
  A File Download dialog box appears.
2. Click Save.
  A Save As dialog box appears.
3. Select a location for the downloaded file, then click Save.
  eArray downloads the information as an Excel file in zip format.

Note: Guest users cannot view bait sequences and statistics.

View bait accessions and annotation

Perform a search for baits.

A search results pane lists the baits that match your search criteria. To navigate among multiple pages of results, use the Pages links.

The accessions associated with each bait appear in the Accessions column in the Search Results pane. Additional annotation, when available, can also appear in the other columns of the search results.

If an accession number appears as a hyperlink, click the link to open the Web site of the relevant database in a new browser window.
To view additional annotation, do any of the following (when available) from the bait search results:

- In the Actions column, next to the bait of interest, click View. The Bait Details page opens in a new window with a list of the available accessions and annotations for the bait.
- In the Chromosomal Location column, next to the bait of interest, click . This opens the Ensembl genome browser in a new window, preset to the specific chromosomal location.
- In the Chromosomal Location column, next to the bait of interest, click . This opens the UCSC genome browser in a new window, preset to the specific chromosomal location.