Overview of mutational strategies |
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When you create a mutagenesis library, eArray asks you to designate the mutational strategy. The mutational strategy refers to the kinds of mutations that you want to generate. eArray offers 3 different strategies that each serve a different purpose.
Mutational strategies create families of sequences that alter codons in the target sequence. You apply mutational strategies on the amino acid level. These mutational strategies are available:
QuikScan 1 – Separately replaces each amino acid in the wild type mutational region with a particular amino acid that you select. For the positions in the mutational region that contain this selected amino acid in the wild type sequence, you can select a different replacement. You can also protect specific positions from changes.
QuikScan 19 – Iteratively replaces each amino acid in the wild type mutational region with all 19 other amino acids, resulting in 19 mutagenic oligos for each amino acid position. You can protect specific positions from changes.
QuikCombine – This strategy accepts a file of amino acid positions and the desired replacement(s) at each position. By default, this strategy makes each requested change individually, but you can also make combinations of changes. You can create all possible combinations of changes at two, three, and/or four positions.
See also