Ways to create mutagenesis libraries

Mutagenesis libraries are collections of DNA oligonucleotides (oligos) that can be used to create variant forms of targeted proteins for a variety of experimental applications (see Learn about mutagenic oligos and mutagenesis libraries). eArray offers two different wizards for creating mutagenesis libraries, which are summarized in the table below. Click the name of each wizard to go to a help topic that describes it in detail.

Method

Comments

Create mutagenesis library from target sequence (wizard)

This wizard leads you through the creation of a custom mutagenesis library based on a single target DNA sequence that you enter. You select regions within the target sequence to which to apply mutations, and you define one or more mutational strategies for each region.

This is a good method to use if you want create mutations in one or more regions of a single 50 kb or smaller target sequence.

Create mutagenesis library from existing oligo sets (wizard)

This wizard leads you through the creation of a custom mutagenesis library comprised of previously-created oligo sets that already exist in your eArray workgroup. To select oligo sets for the library, the wizard lets you search oligo sets by name.

This is a good method to use if you want to create a new library from oligo sets that have already been created and are available in your eArray account.