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Define Design
In this step, complete the fields described below to define the design.
Design Name
Type a name for your design into the field. Alphanumeric characters, hyphens, underscores, and spaces are permitted. The name must be unique within your workgroup.
Species
Specify the species of the targets. For SureSelect Cancer CGP designs, the only available selection is H. sapiens.
Build
The only genome build currently available for SureSelect Cancer CGP designs is hg38. That build is indicated below the Species field.
Create In
Specify the folder in which you want to save this design. The default selection is the top-level folder for your workgroup.
To change the selection, click Select to open the Select Folder dialog box, and mark the folder in which you want to save the new design. This dialog box lists the available folders within your workgroup and, if you are a member of any collaborations, lists the collaboration folders to which you have access. (If you later decide you want to change the folder location of the design, you can move it to another folder.)
TMB Calculation
Mark this check box if you plan to use the design to calculate the TMB (tumor mutational burden) in analyzed samples. When marked, SureDesign provides a warning notification if you attempt to finalize a design that does not contain sufficient exonic content to enable TMB calculation.
MSI Detection
Mark this check box if you plan to use the design to detect MSI (microsatellite instability) in analyzed samples. When marked, SureDesign adds the Agilent MSI Probegroup to the design. This Probegroup covers 288 microsatellite sites and 50 kb of targets.
Description
(Optional) Type a description of the design. The description can be up to 2000 characters. Use only alphanumeric characters (a-z, A-Z, and 0-9) and the following special characters: _, -, $, #, @.
Keywords
(Optional) Type one or more keywords for the design, separated with a comma or space. You can type up to 2000 characters in this field. Use only alphanumeric characters (a-z, A-Z, and 0-9), commas, dashes, and underscores in your keywords. You can later use these keywords to search for the design.
Click Next to advance to the Add/Review
Content step.