About Simple Tiling

Simple Tiling creates Expression type probes that span uploaded target sequence(s) at even intervals. To specify target sequences, you upload a FASTA format file that contains the sequence data.

You can create the tiled probes in several ways:

Average Probe Spacing – Generates evenly-spaced probes that span each sequence with a specified distance between each probe. With this option, targets of different lengths generate different numbers of probes. Because of repeat regions, the actual spacing between probes may deviate from the ideal average probe spacing.
Number of Probes per Sequence – Generates a specified number of evenly-spaced probes for each sequence in your uploaded file. With this option, eArray tiles targets of different lengths at different densities. eArray uses the number of probes per sequence to calculate average probe spacing on a per-interval basis. The actual number of probes designed for each interval can deviate from the specified value, because of repeat regions and rounding, but this deviation should not be large unless sequence length limits the design process.
Total Number of Probes – Generates a specified number of probes, spaced evenly over all of the sequences in your uploaded target sequence file. eArray first calculates the number of probes to be generated for each sequence, and then uses these numbers to calculate average probe spacing.

You can also specify custom probe lengths from 45-60 bp.

After you set up a simple tiling job, you submit it to Agilent for processing. When eArray finishes your simple tiling job, you can view and download the results, and create a probe group based on the results. You can also delete the job.

Note:
• Simple Tiling is not available to guests, or within a collaboration.

• eArray also has tiling tools for other application types. See About Genomic Tiling, Bait Tiling (SureSelect Target Enrichment), and Bait Tiling (SureSelect RNA Enrichment). In addition, you can do an HD Search to retrieve probes from the Agilent HD Probe Database, which contains HD-CGH and HD-ChIP probes that cover the genomes of several species at very high densities.