Overview of SureSelect enrichment libraries |
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The SureSelect enrichment libraries that you can create in eArray let you isolate specific nucleic acid molecules for sequencing. You can create custom libraries of biotinylated RNA "baits" that are complementary to regions of the desired target molecules. The baits capture the desired target molecules by hybridization. Because bait sequences are based on known sequences, these libraries are best used in applications where the underlying sequences are well-characterized, such as in targeted resequencing of genomic DNA that uses next-generation sequencing technology.
eArray has two different SureSelect Enrichment application types: Target Enrichment and RNA Enrichment. Select the application type for your experiements based on the kind of library you want to create:supports these types of enrichment libraries:
SureSelect Target Enrichment Libraries – This type of library, available within the SureSelect Target Enrichment application type, lets you isolate specific genomic DNA fragments of interest. For information about this type of library, see Learn about SureSelect Target Enrichment libraries.
SureSelect RNA Enrichment Libraries – This type of library, available within the SureSelect RNA Enrichment application type, lets you isolate specific RNAs of interest from a larger pool of RNAs. When this type of library is used to enrich an RNA sample, the relative abundance of the enriched RNA species is preserved. For more information about this type of library, see Learn about SureSelect RNA Enrichment libraries.
Within these two main types of libraries, you can work with several specific types of libraries:
Agilent Catalog libraries – These libraries, available to all registered eArray users, have been designed and tested by Agilent. To see the catalog libraries that are available, do a library search, and select AgilentCatalog as the folder to search.
Custom libraries – These libraries are the ones that you can create in eArray that include the bait content of your choice. You can include baits that already exist in eArray, or those that you upload. You can also create and include new baits based on the tiling of specific transcript sequences or genomic regions of interest.
Normal libraries – "Normal library" is the label that eArray assigns to an Agilent Catalog or custom library if it is not a base library, a combined library, or a custom library set, or (for the SureSelect Target Enrichment application type) a supplemental library. See the definitions below.
Base libraries – A base library is an Agilent Catalog library to which you can add baits for additional transcripts or genomic regions when you create a combined library. Only certain Agilent Catalog libraries can be used as base libraries.
Supplemental libraries – A supplemental library contains
baits that you want to add to a given base library to create a combined
library. This gives you a way to add coverage for regions that
may not be represented in the base library.
For the SureSelect Target Enrichment application type, a supplemental
library is a special library with a library type of Supplemental
that was created with the Bait
Tiling wizard. When you create a supplemental library, you select
a specific base library, and eArray uses the same Bait Tiling parameters
that were used to design the baits in that base library.
Note: For combined libraries in the SureSelect RNA Enrichment application type, the supplemental library can be any custom normal library with a status of Complete or Submitted to which you have access in eArray.
Combined libraries – A combined library contains a selected Agilent Catalog library (the base library) and an additional library (the supplemental library). This lets you add baits for genomic regions or transcripts that may not be included in the catalog library.
Custom library sets – (SureSelect Target Enrichment application type only) When you design a custom library that contains more baits than can be accommodated in a single library, eArray automatically creates a custom library set. The library set contains as many individual libraries as are needed to accommodate the requested baits. See Library sets.
For both SureSelect Target Enrichment and SureSelect RNA Enrichment libraries, you can order a library kit that contains the baits in a selected custom library. Specifically for SureSelect Target Enrichment libraries, Agilent also offers specialized SureSelectXT kits. These kits offer a complete Target Enrichment workflow for next generation sequencing. See SureSelectXT kits.
See also