Request quote for library/Buy online |
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You can use eArray to request a quote from Agilent for SureSelect Enrichment kits that are based on the libraries that you can access in eArray. The process is the same for libraries in both individual and collaboration workspaces. You can also go to the Agilent Online Store from within eArray to order kits. You do not need to request a quote for a kit before you order it.
For the SureSelect Target Enrichment application type, you can also use the procedure in this topic to order or request a quote for a library set. You can order or request a quote for the entire library set; however, eArray does not support quotes or orders for individual libraries from within a library set. Also for this application type, in addition to standard library kits, Agilent offers specialized SureSelectXT kits, which supply a complete target enrichment workflow for next generation sequencing.
In this help topic:
Before you request a quote or buy online
To
order a library kit from the Agilent Genomics Web site through eArray
You must be a registered user in eArray and have a valid login name and password.
The library must be located in a folder within eArray that you can view.
The
library must have a status of Submitted. Libraries with this status
have been submitted to Agilent Manufacturing.
Click the Workspace tab or enter a collaboration.
Set the application type to SureSelect Target Enrichment or SureSelect RNA Enrichment.
Do one of the following:
Click the Home tab, then find the desired library in the My Libraries pane.
Search or browse for the desired library. In the search results, find the desired library.
In an individual or collaboration workspace, click Libraries > Request Quote. At the top of the page, My Libraries is selected by default, which lets you request a quote for a custom library that you have created. To request a quote for an Agilent Catalog library, select Catalog Libraries. In the Search Results pane, find the desired library.
In the Actions column, next to the desired library, do one of the following:
To request a quote for a standard library kit that includes all of the baits in the library, click Order Library. Note: This is the only option for combined libraries.
(SureSelect Target Enrichment application type only) To request a quote for a SureSelectXT kit, which includes the standard library kit and other reagents that supply a complete target enrichment workflow for next generation sequencing, click Order XT Library. Note: This is the only option for library sets.
In either case, the Order Libraries page appears. The name of the library and its associated ELID(s) (Enrichment Library ID, a unique identifier that Agilent assigns to your library after you submit it) appear under Library Design Information.
Under Order Quantity, enter the desired values. These details appear:
Detail |
Instructions |
Library Quantity |
Type the desired number of SureSelect kits. |
Number of Unique Baits |
(Read-only) The number of distinct bait sequences represented in the library. |
Base Coverage |
(Read-only) If all baits in the library have chromosomal locations defined for them, this statistic is the total number of bases (in Mb) in the genome of interest (or the corresponding transcripts, as appropriate) that are covered by at least one bait in the library. If one or more baits in the library do not have chromosomal locations defined for them, this statistic is an estimate of the number of bases (in Mb) in the genome of interest (or the corresponding transcripts, as appropriate) that are covered by at least one bait in the library. The estimate is based on the number of unique baits in the library, the bait length, and the bait tiling density. Unless otherwise warranted, eArray assumes a bait tiling density of 2x. |
Multiplexing |
If you want to use the library for multiplexing, mark this option. For certain libraries, this option is marked by default, and cannot be cleared. Libraries used for multiplexing include the necessary Illumina indexing primers, and certain other reagents that differ from those in standard SureSelect kits. |
Reaction Size |
Select the desired reaction size. The numbers in the list indicate the total number of captures that you can do with each custom library kit. |
Sequencing Technology |
Select the sequencing technology that you will use to sequence the genomic fragments or RNAs that are retrieved by your bait library. |
Sequencing Protocol |
Select the desired sequencing protocol. eArray lists option(s) based on your chosen sequencing technology. |
Click Next.
A confirmation page appears. eArray uses the properties of the library
and the details that you entered on the previous page to create the
appropriate part number, and to select the appropriate option numbers.
Do any of the following:
To
change the quote request, click Back.
You can change any of the quote details that you entered in step
4, above.
To print the page, click the Print button.
To delete the quote request, click Cancel.
To submit the quote request to Agilent,
click Request a Quote.
A success message appears.
Click Close.
A message tells you that your submission is complete.
eArray sends the details of your quote request to your Agilent sales
representative. The representative will promptly respond to your request
and provide you with a quote.
Select the desired library and enter the details and quantity as described above in To request a quote, steps 1–6.
On the confirmation page, click
Add to Cart.
The Agilent Genomics Web site
opens. eArray transfers the details about your requested library,
and the quantity that you entered.
See also