View mutagenesis oligo set

You can view information about a specific mutagenesis oligo set, including the list of oligos in the oligo set with their sequences, and statistical information.

Search for the oligo set that you want to view.

A list of oligo sets appears.
In the Actions column of the list, next to the desired oligo set, click View.

The View Oligo Set page opens in a new window. The top pane contains the following information. All items are read-only, except as noted.

Property	Details
Oligo Set Name	Oligo set names have this format: <target sequence name>_<mutational region>_<mutational strategy>. Example: An oligo set with the name ATPase_30-72_QuikScan1 contains mutagenic oligos that were created by the application of the QuikScan1 mutational strategy to amino acids 30 to 72 of the translated ATPase target sequence.
Mutational Region Name	Mutational region names have this format: <target sequence name>_<mutational region>. Example: The mutational region ATPase_30-72 includes amino acid positions 30 to 72 of the translated target sequence named ATPase. Target sequences are named when they are entered in eArray as part of the creation of mutagenesis oligo sets or libraries.
Mutational Strategy	eArray supports several mutational strategies, which each apply mutations to a given mutational region in a specific way. For details see Mutagenesis libraries.
No. of Oligos	Total number of oligos in the oligo set.
Created by	User who defined the oligo set and saved it.
Created Date	Date the oligo set was first saved in eArray.
Locked	eArray always creates mutagenesis oligo sets with a status of Locked. Locked oligo sets cannot be edited, and they also cannot be unlocked.
Host Codon Preference	To see the codon usage table that was used in the construction of mutant codons for the oligo set, click Show Codons. The codon usage table opens in a new window. The most commonly occurring codon for each amino acid in the selected host type appears in red. Marked codon(s) for each amino acid are the ones that were used to create mutant codons in the oligo set.
5' Primer	The sequence of the PCR primer that is designed for the 5' end of every oligo in the oligo set. PCR primers are later used with the QuikChange Oligo Library Mutagenesis System to amplify the mutagenic oligos in a library.
3' Primer	The sequence of the PCR primer that is designed for the 3' end of every oligo in the oligo set. PCR primers are later used with the QuikChange Oligo Library Mutagenesis System to amplify the mutagenic oligos in a library.

The Search Results pane lists the ID of each oligo in the oligo set, along with the amino acid position in the target sequence that is mutated by the oligo. The nucleotide sequence of each oligo also appears. These oligo sequences only include the part of the oligo that corresponds to the mutational region (the PCR primer annealing sequences are not shown).

Click Close.

See also

Mutagenesis libraries

Mutagenesis oligo sets