Request quote for library

 

You can use eArray to request a quote from Agilent for SureSelect Target Enrichment kits that are based on the libraries that you can access in eArray. The process is the same for libraries in both individual and collaboration workspaces.

Before you request a quote

How to request a quote

  1. Click the Workspace tab or enter a collaboration.

  2. Set the application type to SureSelect Target Enrichment.

  3. Do one of the following:

In each case, the Request Quote page appears. The name of the library and its associated ELID (Enrichment Library ID, a unique identifier that Agilent assigns to your library after you submit it) appear under Library Details.

  1. Under Quote Details, enter the desired values. These details appear:

Detail

Instructions

Quantity

Type the desired number of SureSelect Target Enrichment kits.

Reaction Size

Select the desired reaction size. The numbers in the list indicate the total number of captures that you can do with each custom library kit.

Number of unique Baits

(Read-only) The number of distinct bait sequences represented in the library.

Base Coverage

(Read-only) If all baits in the library have chromosomal locations defined for them, this statistic is the  total number of bases (in mb) in the genome of interest that are covered by at least one bait in the library.

If one or more baits in the library do not have chromosomal locations defined for them, this statistic is an estimate of the number of bases (in mb) in the genome of interest that are covered by at least one bait in the library. The estimate is based on the number of unique baits in the library, the bait length, and the bait tiling density. Unless otherwise warranted, eArray assumes a bait tiling density of 2x.

Multiplexing

Mark this option if you want to use the library for multiplexing. For certain smaller libraries, this option is selected by default, and cannot be cleared. Libraries used for multiplexing include the necessary Illumina indexing primers, and certain other reagents that differ from those in standard SureSelect Target Enrichment kits.

Sequencing Technology

Select the sequencing technology that you will use to sequence the genomic fragments that are retrieved by your bait library.

Sequencing Protocol

Select the desired sequencing protocol. eArray lists option(s) based on your chosen sequencing technology.


  1. Click Next.

    A confirmation page appears, with the details of your quote request. eArray uses the properties of the library and the details that you entered on the previous page to create the appropriate part number, and to select the appropriate option numbers.

  2. Do any of the following:

  1. To submit the quote request to Agilent, click Submit.

    A success message appears.

  2. Click Close.

    A message tells you that your submission is complete.

    After eArray processes your request, you receive a confirmation e-mail from Agilent. An Agilent sales representative will contact you to follow up on the quote.

See also

Submit library

Target enrichment and eArray

Libraries